Multiplexing spectra and lifetime in 32 channels simultaneously
Enrico Gratton and Lorenzo Scipioni
Laboratory for Fluorescence Dynamics. Biomedical Engineering Department. University of California, Irvine, USA
The microscope built allows simultaneous spectral phasors and lifetime phasors. Using these set of data on 7 different dyes we can separate stem cells going through division as well as cells having lipid particles of different sizes in 3D structures such as organoid and skin.